From sequence search to a record you can review.
BLASTseq AI combines live NCBI alignment with position-aware mismatch analysis, hybridization thermodynamics, multiplex interaction checks, and a versioned record of the result.
Why ΔG°T over percent identity
Percent identity describes how many bases match. It does not describe how strongly the resulting duplex is expected to bind. Hybridization ΔG°T adds that thermodynamic context, so a mismatch can be evaluated by its predicted effect on binding rather than treated as equivalent to every other mismatch.
AOAC Appendix Q provides methodological support for using thermodynamic analysis in in silico inclusivity and exclusivity assessment. It is not certification, approval, endorsement, or recognition of BLASTseq AI.
How the composite score is built
Every hit is evaluated on three dimensions: sequence identity, mismatch location impact, and hybridization ΔG°T. These resolve into a single composite call, with the contributing factors shown so you can understand the result.
Mismatch position matters because an oligo does not tolerate every mismatch equally. The production method divides primer binding regions by position and gives the 3′ end the strictest treatment: a mismatch in the terminal five bases can block a detecting call, while mismatches farther from the extension end are evaluated with progressively different tolerances. Probe mismatches are evaluated separately. IUPAC-compatible bases, substitutions, insertions, deletions, and the thermodynamic result all contribute to the final explained call.
How every run is documented
Each report records the reference-database release and search date the analysis ran against, along with your query sequences and scoring parameters. That makes the analysis reproducible and citable months or years later — which is what turns a screening result into a record you can file.
What this supports
For device manufacturers, molecular device classifications require a documented protocol for continuous monitoring of genetic mutations, naming periodic in silico analysis as the method — 21 CFR 866.3981 and 866.3982. For laboratories, establishing performance specifications for an LDT under CLIA requires analytical specificity including interfering substances — 42 CFR 493.1253.